DNA

Part:BBa_K4828006

Designed by: Yota Kashima   Group: iGEM23_TokyoTech   (2023-10-10)


dengue virus1 partial Envelope gene

This is part of the sequence that encodes the envelope protein of dengue virus type 1. This is a dengue virus serotype-specific sequence, and the type-specific gene can be identified and detected by using primers with this sequence as the template in PCR. In PCR, we use GAACATGGAACAACTGCAACCA (BBa_K4828010) as forward primer and CCGTAGTCTGTCAGCTGTATTTCC (BBa_K4828014) as reverse primer.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 85
    Illegal BsaI.rc site found at 108


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Parameters
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